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KMID : 0371019990320020170
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Journal of Preventive Medicine and Public Health
1999 Volume.32 No. 2 p.170 ~ p.176
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A Study on the Protective Effects of Glutathione on Cytotoxicity of Mercury and Cadmium
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Jeong Jae-Ho
Kim Jun-Youn
Koh Dai-Ha
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Abstract
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Objectives: To evaluate the protective effects of glutathione(GSH) on the cytotoxicity of mercurial compounds(CH₃HgCl, HgCl₂) or cadmium chloride(CdCl₂) in EMT-6 cells.
Methods: The compounds investigated were CH3HgCl, HgCl₂, CdCl₂, GSH, buthionine sulfoximine(BSO), L-2-oxothiazolidine-4-carboxylic acid(OTC). Cytotoxicity analysis consist of nitric oxide(NO) production, ATP production and cell viability.
Results: Mercurial compounds and cadmium chloride significantly decreased cell viability and the synthesis of NO and cellular ATP in EMT-6 cells. GSH was not toxic at concentrations of 0 - 1.6 mM. In the presence of GSH, mercurial compounds and cadmium did not decrease the production of ATP and nitrite in EMT-6 cells. The protective effects of GSH against the cytotoxicity of mercurial compounds and cadmium depended on the concentration of added GSH to the culture medium for EMT-6 cells. We evaluated the effects of intracellular GSH level on mercury-or cadmium-induced cytotoxicity by the pretreatment experiments. Pretreatment of GSH was not changed NO₂- and ATP production, and pretreatment of BSO was decreased in dose-and time-dependent manner. Pretreatment of OTC was increased NO₂- and ATP production in dose- and time-dependent manner. Because intracellular GSH level was increased by OTC pretreatment, the protective effect on mercury-and cadmium-induced cytotoxicity was increased.
Conclusions: These results indicated that sulfhydryl compounds had the protective effects against mercury-induced cytotoxicity by the intracellular GSH levels.
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KEYWORD
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Glutathione, Mercury, Cadmium, EMT-6, Nitric Oxide
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